Verification of column agglutination technology with conventional tube technology for naturally occurring antibody titration
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چکیده
منابع مشابه
Conventional tube agglutination with polyethylene glycol versus Red Cell Affinity Column Technology (ReACT): a comparison of antibody detection methods.
A procedure for antibody detection and identification that utilizes affinity microcolumns to isolate IgG antibodies in a gel matrix containing Protein G and Protein A was commercially available in recent years. We evaluated this method (ReACT, Red Cell Affinity Column Technology, Immucor Co., Norcross, GA) as an alternative to standard tube agglutination testing, in an effort to minimize subjec...
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INTRODUCTION Measurement of alloantibody titer to a red cell antigen (ABO titers) is an integral part of management of ABO incompatible kidney transplants (ABOiKT). MATERIAL AND METHODS There are different methods of titer estimation. Alloantibody detection by tube titration and Gel agglutination columns are accepted methodologies. It is essential to find the difference in titers between the ...
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Anti-D immunoglobulin; BioVue column agglutination test; Quality control; Haemolytic disease; Anti-Rh(D) titration; Rh-factor Abstract The preparation anti-D immunoglobulin is an important therapy for both treatment and protection. Titer of anti-D immunoglobulin preparation is an important factor to determine the dose of treatment. Many methods are used to evaluate the titer of anti-D immunoglo...
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IT IS accepted generally that antibodies directed against red blood cell antigens in the Rh system result from isoimmunization either by transfusion or pregnancy and rarely can be considered “naturally-occurring.” Diamond et al. reported the first example of a naturally-occurring antibody within this system in 1942.3 They described an antibody, apparently anti-D, in the serum of a five-year-old...
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Agglutinating substances having characteristics of naturally occurring macroglobulin antibodies to human Bence Jones proteins have been identified in human sera. By means of hemagglutination and hemagglutination inhibition techniques, common determinants have been demonstrated on the light (L) polypeptide chains of pooled normal human gamma(2)-globulin and on some Bence Jones proteins of group ...
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ژورنال
عنوان ژورنال: Global Journal of Transfusion Medicine
سال: 2016
ISSN: 2455-8893
DOI: 10.4103/2455-8893.189846